Coding

Part:BBa_K184001:Design

Designed by: Jennifer Lei   Group: iGEM09_Bay_Area_RSI   (2009-10-14)


Silintaphin-1 + gs linker partB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 19
    Illegal BsaI.rc site found at 142
    Illegal SapI.rc site found at 61
    Illegal SapI.rc site found at 154


Design Notes

Synthetic gene assembly of Silintaphin-1 was created by splitting the Silintaphin-1 gene into (2) separate parts because of high GC content and repeat sequences introduced by the (GS4)6 linker.


Source

Silintaphin-1 was created by synthetic gene synthesis with silent mutations to delete biobrick cloning sites in the endogeneous gene sequence. The gene was also codon optimised for human and e.coli expression. The Silintaphin-1 gene was derived from the silicanaceous sponge, Suberites domuncula.

References